This Technical Report specifies three methods for the detection and enumeration of Escherichia coli in sludge, treated biowaste and soil: ? Method A - Membrane filtration method for quantification (see Clause 6); ? Method B - Miniaturised method (Most Probable Number, MPN) by inoculation in liquid medium (see Clause 7); ? Method C - Macromethod (Most Probable Number) in liquid medium (see Clause 8).